Ruprecht-Karls-Universität Heidelberg

Overview Electrophysiology Nonlinear Microscopy Calcium Imaging Modelling Image Processing Single Molecules

Tissue engineering

Figure 1: 6-well cell-culture plates with PDMS molds and cutouts
Figure 2 Contractile force measurement setup with a myoid mounted

3-D Myooid Tissue Engineering to reduce and replace the use of animal model in muscle research in relevance of Duchenne Muscular Dystrophy

It is well known the modulatory effect of NO in the Ca2+ homeostasis in skeletal muscle under physiological conditions. The mdx mouse model for Duchenne Muscular Dystrophy (DMD) has been used to show that muscle activity can be improved once the correct function of NO and its signal pathway is restored. However, it is unknown the mechanisms underlies the action of NO. Common issues of animal research, such as ethical problems, high cost of animal housing and limited amount of tissue per animal lead us to design a new strategy using the C2C12 cell line to tissue engineer (TE) muscle fibers for the use in DMD research.

Therefore, our goal is to use simultaneous recordings of Ca2+-transients and force production from myooids to be used for complete molecular and physiological characterization of ECC to suggest an alternative and new method replacing the mouse animal model.

In this proposal, the myooid technique will completely REPLACE the use of the DMD mouse model, REDUCING the future use of animals in basic and translational research. Developing the 3D myooid TE will allow us to evaluate the effect of NO on simultaneous recording of force production and Ca2+-transients the most relevant aspects of the ECC in dystrophic muscles.

 

Project Funded by Bundesministerium für Bildung und Forschung (BMBF) 031A552

 

Dr. Matias Mosqueira

Universität Heidelberg,

Institut für Physiologie und Pathophysiologie

Im Neuenheimer Feld 326, R414

69120 Heidelberg

Deutschland